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KMID : 0379519990150030331
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한국독성학회지
1999 Volume.15 No. 3 p.331 ~ p.337
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Cell Isolation Using Centrifugal Elutriation System
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Yoon Byong-Ill
Kim Dae-Yong
Lee Jae-Hyun
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Abstract
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Viability, purity and recovery rate are the most important criteria that we should consider when we attempt to isolate cells. Centrifugal elutriation is a such technique designed to separate various cells according to their size. Centrifugal elutriation is a relatively gentle means of separating cells since this method can precludes the cells from the deleterious effects of pelleting or osmotic effects of density gradient centrifugation during separation, otherwise at least alleviate such harmful effects and relatively shorten the separation times by decreasing the frequency of pelleting or gradient centrifugation. In addition, purity as high as 95 to 97% and 85 to 97% recovery rate can be easily achieved by centrifugal elutriation. In this paper, we describe the principle, method, and applicability of elutriation centrifuge system and hope we share our information and experience with researchers who are working in the field of cell isolation.
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KEYWORD
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Cell isolation, centrifugal elutriation
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